Thursday, February 26, 2009

delays in posting: Ti runAnalysisPipe issues

Well, I've been up to my ears in getting the runAnalysisPipe to do the signal processing on our most recent Ti run. The good news is we got ~1.5 million reads but the bad news is all of that data is tied up in the CWF format....
For those interested, here is the verbose output. it seems to be kicking out of the program with no error. Roche has assigned a GC number to it so hope to post that soon.
----gsRunProcessorlog-------
[Wed Feb 25 09:02:21 2009][Debug][] Logging configured.
[Wed Feb 25 09:02:21 2009][Information][] gsRunProcessor 2.0.00.22 (Build 184) Starting
[Wed Feb 25 09:02:21 2009][Debug][] Parsing pipeline: /etc/gsRunProcessor/signalProcessing.xml
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step NukeSignalStrengthBalancer (pass 1)
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step BlowByCorrector
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step CafieCorrector
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step NukeSignalStrengthBalancer (pass 2)
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step IndividualWellScaler
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step MostLikelyErrorSubtractor
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step WellScreener (pass 1)
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step MetricsGenerator
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step QualityFilter
[Wed Feb 25 09:02:21 2009][Debug][ProcessingEngine] Adding step BaseCaller
[Wed Feb 25 09:02:29 2009][Information][] Detected processor speed: 2129 MHz.
[Wed Feb 25 09:02:39 2009][Notice][ProcessingEngine] Starting job eb6dd82e-0344-11de-ad6c-0010182f8ff4.
[Wed Feb 25 09:02:39 2009][Debug][ProcessingEngine] Creating 1 processing group(s).
[Wed Feb 25 09:02:39 2009][Information][ProcessingEngine] Using memory-only storage for flowgrams.
[Wed Feb 25 09:02:39 2009][Notice][ProcessingEngine] Processing Group 0 : Loading data.
[Wed Feb 25 09:02:39 2009][Information][ProcessingEngine] Opening file /home/kc/Desktop/D_2009_02_20_12_47_04_FLX02070135_imageProcessingOnly/regions/2.cwf
[Wed Feb 25 09:02:39 2009][Debug][ProcessingEngine] Region 2 : Process 0 is loading 2049,1 4095,4095

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