new review paper

I wrote a review on metagenomics and high-throughput sequencing for Estuaries and Coasts. It highlights a series of pitfalls that I've seen over the last two years doing Roche/454 runs. You can find a preprint here.

STOP! Important notice inside

I never like seeing stickers like this on Roche/454 reagents. Turns out there is a new "additive" to the emPCR for Titanium. More can be read here.

swine flu was around in 2008

An advance paper in Nature on the origin and TMRCA of the 2009 swine flu.

bTEFAP vs Sogin et al barcodes?

Has anyone every tried the bTEFAP protocol for barcoding amplicons for FLX runs? If so, please let me know... All amplicons we've done to date have been similar to Sogin's protocol for barcoding.

Bee parasite genome released

A new paper on a parasite implicated in Colony Collapse Disorder in bees. Here is the press release.

Closing gaps in the human genome with 454

You never finish a genome.... Here is a group from MIT/Broad with a new paper in Genome Biology on closing gaps (class IIIs) in the human genome with 454.

Gene expression using 454 data

THe first paper on using 454 data for gene expression studies.