Barcoding for the masses

Many people want to use the barcoding option (=MIDs) for pyrosequencing but either 1) want to sequence more than 12 different samples at one time (the current number available from Roche), 2) want to use Titanium (which currently does not have available the emulsion kits for the barcoding), or 3) want to do it themselves. A protocol from Matthias Meyer and colleagues provides the details for those interested in doing their own barcoding in their extremely detailed paper "Parallel tagged sequencing on the 454 platform" in Nature Protocols (http://www.nature.com/nprot/journal/v3/n2/abs/nprot.2007.520.html). The software is extremely straightforward to use as well.